anti-cav1.2a (cacna1c) antibody (Alomone Labs)
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Anti Cav1.2a (Cacna1c) Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cacna1c/Anti-CaV1%2E2a+(CACNA1C)+Antibody/custom%40acc-013%4042203078
Average 94 stars, based on 13 article reviews
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Membrane:Article Title: Enhancement of airway ciliary beating mediated via voltage-gated Ca 2+ channels/α7-nicotinic receptors in mice. Article Snippet: Acetylcholine (ACh), which activates muscarinic ACh receptors (mAChRs) and nicotinic ACh receptors (nAChRs), enhances airway ciliary beating by increasing the intracellular Ca2+ concentration ([Ca]i).. The mechanisms enhancing airway ciliary beating by nAChRs have remained largely unknown, although those by mAChRs are well understood.. In this study, we focused on the effects of α7-nAChRs and voltage-gated Ca2+ channels (CaVs) on the airway ciliary beating. Incubation:Article Title: Variable Contribution of TMEM16A to Tone in Murine Arterial Vasculature. Article Snippet: .. Membranes were incubated overnight at 4°C in blocking buffer with primary antibody either against TMEM16A (1:500; Abcam #ab53212, UK), or Article Title: Ion Channel Dysfunctions in Dilated Cardiomyopathy in Limb-Girdle Muscular Dystrophy Article Snippet: .. iPSC-CM were seeded in matrigel-coated 8-well Lab-Tek chambers (BD Biosciences) and incubated with the following primary antibodies at a dilution of 1:200: α-actinin (Sigma Aldrich), troponin T type 2 (Sigma Aldrich), Article Title: Ion Channel Dysfunctions in Dilated Cardiomyopathy in Limb-Girdle Muscular Dystrophy Article Snippet: .. iPSC-CM were seeded in matrigel-coated eight-well Lab-Tek chambers (BD Biosciences) and incubated with the following primary antibodies at a dilution of 1:200: alpha-Actinin (Sigma Aldrich), TNNT (Sigma Aldrich), Blocking Assay:Article Title: Variable Contribution of TMEM16A to Tone in Murine Arterial Vasculature. Article Snippet: .. Membranes were incubated overnight at 4°C in blocking buffer with primary antibody either against TMEM16A (1:500; Abcam #ab53212, UK), or Control:Article Title: Variable Contribution of TMEM16A to Tone in Murine Arterial Vasculature. Article Snippet: .. Membranes were incubated overnight at 4°C in blocking buffer with primary antibody either against TMEM16A (1:500; Abcam #ab53212, UK), or Article Title: Embryo-derived trypsin-induced calcium entry is inhibited by endometrial infertility factor, LEFTY2 Article Snippet: .. Membranes were probed overnight at 4°C with antibodies: LEFTY2 (1:500, sc-365845, Santa Cruz, Germany), Western Blot:Article Title: Loss of connectin novex-3 leads to heart dysfunction associated with impaired cardiomyocyte proliferation and abnormal nuclear mechanics. Article Snippet: .. :(0123456789) Scientific Reports | (2024) 14:13727 | https://doi.org/10.1038/s41598-024-64608-1 membranes were blocked with 5% nonfat milk in TBS/T, probed with primary antibodies for novex-3 (TTN2, Myomedix, designated as ab1 in Fig. 1a)16, connectin N-terminus (raised in our lab, designated as ab3 in Fig. 1a)44, Aurkb (A5102, Sigma-Aldrich), Nusap1 (12024-1-AP, Proteintech), Ccna2 (ZRB1590, Sigma-Aldrich), Atp2a2 (MA3-919, Thermo-Fisher), Article Title: Atrial cardiomyocytes contribute to the inflammatory status associated with atrial fibrillation in right heart disease Article Snippet: CACNA1C, CX43, IL1β, IL6, KCNQ1, NLRP3, PLN, RYR2, SCN5A, TGFβ1, and GAPDH qPCR probes as well as DAPI and WGA were obtained from Invitrogen (Waltham, MA, USA). .. Primary antibodies for immunoblot experiments included: BIN1 (Novus Biologicals, NBP1-89102), Article Title: Loss of connectin novex-3 leads to heart dysfunction associated with impaired cardiomyocyte proliferation and abnormal nuclear mechanics Article Snippet: The samples were then transferred onto nitrocellulose (Bio-Rad) or PVDF (GE Healthcare, USA) membranes using HorizeBLOT 2 M (WSE-4025, ATTO Corporation, Japan) at 20–25 V constant for 10–45 min, Trans-Blot Turbo (Bio-Rad) at 2.5 A constant for 3 min, or in the transfer buffer (25 mM Tris, 192 mM glycine and 20% methanol supplemented with or without 10 mM β-mercaptoethanol or 1% SDS) at 0.07–0.15 A constant for overnight. .. The membranes were blocked with 5% nonfat milk in TBS/T, probed with primary antibodies for novex-3 (TTN-2, Myomedix, designated as ab1 in Fig. a) , connectin N-terminus (raised in our lab, designated as ab3 in Fig. a) , Aurkb (A5102, Sigma-Aldrich), Nusap1 (12024-1-AP, Proteintech), Ccna2 (ZRB1590, Sigma-Aldrich), Atp2a2 (MA3-919, Thermo-Fisher), |
